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. 2017 Jul 20;7:5986. doi: 10.1038/s41598-017-06236-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Antigen uptake by monocytes, migratory DCs and resident DCs in lymph nodes, (A) Gating strategy applied to identify OVA-AF647+ Ly6C^hi monocytes, migratory DCs and resident DCs in the draining lymph nodes of OVA-AF647/CpG injected mice. (B,C) Graphs showing respectively the percentages (B) and absolute numbers (C) of Ly6C^hi monocytes, migratory DCs and resident DCs that have acquired OVA-AF647 in the draining lymph nodes at the indicated time intervals post OVA-AF647 injection (n = 4). Data are representative of three independent experiments.