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. 2017 Jul 20;7:5986. doi: 10.1038/s41598-017-06236-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Migratory DCs constitute the major antigen presenting population to CpG adjuvanted protein vaccination. (A) Proliferation of CFSE labelled OT-I and OT-II cells following incubation with respectively Ly6C^hi monocytes, migratory DCs and resident DCs sorted from the pooled draining lymph nodes of 30 mice injected with OVA/CpG. (n = 5; mean +/− SD). Data shown are representative of two independent sorting experiments. (B–C) Flow cytometric comparison of Ly6C^hi monocyte and migratory DC influx in the draining lymph nodes of CpG injected WT and Ccr7^−/− mice. (B) Contour plots showing Ly6C^hi monocyte and migratory DC frequencies.(C) Graph showing the absolute numbers of Ly6C^hi monocytes and migratory DCs (n = 5; mean +/− SD). (D–E) Flow cytometric analysis of OT-I proliferation in the draining lymph nodes of WT and Ccr7^−/− mice immunized four days earlier with OVA/CpG. (D) Histogram overlay of OT-I proliferation in WT and Ccr7^−/− mice. (E) Graph quantifying the total numbers of OT-I cells and the number of OT-I cells having undergone over 4 divisions (n = 5; mean +/− SD). Data are representative of three independent experiments.