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. 2017 Jul 21;10:226. doi: 10.3389/fnmol.2017.00226

Figure 1.

Figure 1

Thalamic hemorrhagic rats exhibit bilateral mechanical pain hypersensitivity and microglial and astrocytic activation in peri-thalamic lesion sites. (A) Photomicrograph of brain slice showing the hemorrhagic lesion site in the thalamus following ITC. Scale bar, 1 mm; ic, internal capsule; Po, posterior thalamic nuclear group; VPL, ventral posterolateral nucleus of the thalamus; VPM, ventral posteromedial nucleus of the thalamus. (B) Development of bilateral mechanical pain hypersensitivity induced by ITC. Saline injection served as control. ITS, intra-thalamic saline; ITC, intra-thalamic collagenase injection; contra, contralateral; ipsi, ipsilateral; PWMT, paw-withdrawal mechanical threshold; ***P < 0.001 ITC-ipsi vs. ITS-ipsi; ###P < 0.001 ITC-contra vs. ITS-contra; n = 10 rats/group. (C) Representative immunofluorescent photomicrographs showing the time course expression of Iba-1 (red) and GFAP (green), markers of microglia and astrocytes respectively, in the peri-thalamic lesion sites. The hemorrhagic lesion core is on the right side of the white line in each image. Scale bar, 400 μm. (D) Iba-1 and GFAP expression as examined using Western blot assay. Representative bands are shown on the top, and data summary is shown on the bottom. ***P < 0.001 vs. ITS-7 d group; n = 4/group.