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. 2017 Jul 21;10:226. doi: 10.3389/fnmol.2017.00226

Figure 2.

Figure 2

Effects of intra-thalamic injection of minocycline and fluorocitrate on Iba-1 and GFAP expression and the established central post-stroke pain (CPSP). (A) Representative immunofluorescent photomicrographs showing expression of Iba-1 and GFAP in peri-thalamic lesion sites following intra-thalamic minocycline (10 μg, 1 μl) and fluorocitrate (1 nM, 1μl) injection. The hemorrhagic lesion core is on the right side of the white line in each image. Scale bar, 400 μm. (B) Quantification of the mean immunofluorescent intensity of Iba-1 and GFAP following intra-thalamic minocycline and fluorocitrate injection. ***P < 0.001, ITC-Mino vs. ITC-Veh; ###P < 0.001, ITC-Fluo vs. ITC-Veh; n = 4/group. (C) Temporal pattern of effects of intra-thalamic minocycline and fluorocitrate injection at 10 days post thalamic hemorrhage on the established bilateral mechanical pain hypersensitivity. PWMT, paw-withdrawal mechanical threshold; *P < 0.05, **P < 0.01, ***P < 0.001, ITC-Mino vs. ITC-Veh; #P < 0.05, ##P < 0.01, ###P < 0.001, ITC-Fluo vs. ITC-Veh; n = 8/group.