Figure 9.

Effects of intra-thalamic injection of CoCl₂ on HIF-1α, SDF1 and CXCR4, as well as on microglial marker Iba-1 and astrocytes marker GFAP expression in naïve rats. (A) Representative bands showing the effect of intra-thalamic injection of CoCl₂ (100 mM, 1 μl) on HIF-1α, SDF1, CXCR4, as well as on Iba-1 and GFAP expression in the thalamic injection sites. (B) Quantification of Western blot assay showing that intra-thalamic CoCl₂ injection increased HIF-1α, SDF1 and CXCR4 expression, and which were prevented by pre-injection of AMD3100 and YC-1. ^£P < 0.05, ^££P < 0.01, ^&&&P < 0.001, ***P < 0.001 vs. ITS-7 d; ^§§P < 0.01, ^§§§P < 0.001, ^#P < 0.05, ^###P < 0.001, ^¥P < 0.05 vs. IT-CoCl₂ at corresponding time points; n = 5/group. (C) Quantification of Western blot assay showing that intra-thalamic CoCl₂ injection increased Iba-1 and GFAP expression, and which were prevented by pre-injection of AMD3100 and YC-1. *P < 0.05, ***P < 0.001, ^&&&P < 0.001 vs. ITS group; ^§§P < 0.01, ^§§§P < 0.001, ^###P < 0.001, vs. IT-CoCl₂ at corresponding time points; n = 5/group. (D) Intra-thalamic injection of CoCl₂ induced bilateral mechanical pain hypersensitivity in naïve rats which were prevented by pre-injection of AMD3100. *P < 0.05, **P < 0.01, IT-CoCl₂/AMD vs. IT-CoCl₂/Veh; PWMT, paw-withdrawal mechanical threshold; n = 7/group.