Figure 1.

In silico comparison of gene dysregulation in super oxide dismutase 1 (SOD1)^G93A microglia. Data compared are from the original publications of Chiu et al. (2013) and Noristani et al. (2015). Venn diagrams showing that 45 genes are commonly dysregulated in SOD1^G93A microglia at all time points in both studies, corresponding to 0.9% of the dysregulated genes. Brca1 is amongst these 45 genes. A total of 5117 genes were identified as dysregulated, 621 genes are uniquely dysregulated in hSOD1^G93A microglia at P65, 408 at P90, 816 at P100, and 1072 at P130. 1.17% of the identified genes are commonly dysregulated at P65 and P90; 4.4% are commonly modified at P90 and P100 and, 15% are commonly dysregulated at P100 and P130. In the study of Chiu et al. (2013) microglia were isolated by FACS from the whole SOD1^G93A spinal cord and RNA sequencing (RNA-Seq) was used. In the study of Noristani et al. (2015) microglia were isolated by FACS from the lumbar segment of the SOD1^G93A spinal cord and microarrays were used. P65 correspond to onset/early symptomatic phase, P90 early symptomatic, P100 symptomatic and P130 the end stage of the disease. Thresholds to select the genes were identical in the two studies with a fold change >2 and p value (FDR) <0.05.