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. 2017 Jul 20;7:5987. doi: 10.1038/s41598-017-05553-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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TNF-α secretion by γδ T cells stimulated with tumor cells pretreated with BP prodrugs and their acids. TNF-α production by γδ T cells in response to EJ-1 bladder cancer cells pretreated with various concentrations of bisphosphonate prodrugs was compared with that in response to EJ-1 bladder cancer cells pretreated with their corresponding acids: (A) compounds 3 (⚫) and 10 (⚪), (B) 5 (⚫) and 12 (⚪), (C) 6 (⚫) and 13 (⚪), (D) 7 (⚫) and 14 (⚪).