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. 2017 Jul 20;7:5976. doi: 10.1038/s41598-017-05156-9

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(a) Confocal fluorescence microscope images of fixed NIH/3T3 fibroblast cells co-stained with Ag/Au-t clusters (blue) and CyTRAK-26 dye (red) under UV excitation at increasing cluster concentration (7 µM and 33 µM). (b) Cytotoxicity test and (c) ROS concentration level test on NIH/3T3 cells stained with 7 µM of Ag, Ag/Au-t and Au clusters, taken at four time-points during cells proliferation (24 h, 48 h, 72 h and 96 h). (d) Cellular proliferation test on cells in artificially stressed culture conditions by adding the metabolic accelerator menadione. The histogram shows the incremental cell proliferation of cells stained with the metal clusters calculated with respect to the unstained control culture.