Figure 3.

Binding of Affibody molecules to various Aβ aggregates and monomer in a batch mode affinity experiment. The SDS-PAGE analysis shows that Affibody Z_{A β 42cc_1} (a) and Z_{A β 42cc_4} (c) recognize protofibrillar aggregates of Aβ₄₂cc and Aβ₄₂wt (bound Aβ). Wild type monomer and fibrils did not bind to the Affibody molecules and were recovered in the supernatant (non-bound Aβ). The designations MW, CC-PF, WT-M, WT-PF and WT-F refer to molecular weight marker (GE Healthcare), Aβ₄₂cc protofibrils, Aβ₄₂wt monomer, Aβ₄₂wt protofibrils and Aβ₄₂wt fibrils, respectively. (b) and (d), Aβ band intensity for Z_{A β 42cc_1} and Z_{A β 42cc_4}, respectively. The band intensities were obtained with the graphic program ImageJ³². The data was normalized with respect to each Aβ-species, i.e. the sum of the intensities for bands originating from one Aβ-species is equal to 100%.