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. 2017 Jul 20;7:5952. doi: 10.1038/s41598-017-06416-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Simulation of microgravity induces cell clumping. The colorectal cancer cells DLD1 (a), HCT116 (c) and SW620 (e), when cultured in Rotary cell culture system at 10 RPM for 48 hours to simulate microgravity clump together to form large 3D structures (b,d,f) DLD1, HCT116 and SW620 respectively. Scale bar represents 200 μm. XTT assay of the microgravity simulated cells (g) shows the cell viability was significantly reduced. The experiment was performed thrice with individual controls **P < 0.005, *P < 0.05. Data represented as mean + S.D.