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. 2017 Jul 21;8:1378. doi: 10.3389/fmicb.2017.01378

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Copyright © 2017 Li, Cui, Yang, Cui, Wei, Wu, Wang and Zhou.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Phylogenetic tree under maximum likelihood criteria of FeOB-associated OTUs (bold) detected from our present study together with other known iron-oxidizing bacterial species and environmental clones (Emerson and Moyer, 2002; Kato et al., 2009, 2014; Li et al., 2012). Sequences of 16S rRNA gene obtained from universal bacterial primers 21F/1492R (∼1.5 kb) and FeOB-specific primers Zeta674F/1492R (800 bp), 122F/998R (900 bp) are combined together to construct the phylogenetic tree. Numbers in brackets indicate the number of clones. Bootstrap support is shown at nodes for 1000 bootstrap replicates under maximum likelihood.