Figure 2.

Functional characterization of mouse α9α10 nAChRs heterologously expressed in Xenopus laevis oocytes. Oocytes expressing mouse α9α10 nAChRs were subjected to two-electrode voltage clamp electrophysiology as described in Materials and Methods. The EC₅₀for activation of α9α10 nAChRs by ACh was 30.1 (23.5–38.7) μM and the Hill slope was 1.1 (0.8–1.4) (n = 5). The EC₅₀ for activation by choline was 601 (311–1160) μM; Hill slope 1.3 (0.5–2.1) maximal efficacy was 50 ± 5% relative to ACh (n = 4). Nicotine failed to evoke currents in all oocytes tested (0.4 ± 0.2%) (n = 4). The values in parenthesis denote the 95% confidence interval.