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. 2017 Jul 20;7:5964. doi: 10.1038/s41598-017-06549-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Immunohistochemical staining of tissue sections derived from the organ block of the transparent pregnant uterus. (a) The procedure of immunohistochemical staining of the tissue sections derived from the organ block of the transparent pregnant uterus. After the target sections, presented as a white square in the light-sheet image (b), were obtained with cryostat, immunohistochemical staining was performed with anti-CD31 (green) and anti-cytokeratin-7 (purple) antibodies (c and d). Note that trophoblast giant cells and decidual vessels are determinately visible at the feto-maternal interface. TG, trophoblast giant cells. Scale bars, 1 mm (b) and 100 µm (c and d).