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. 2017 Jul 20;7:6015. doi: 10.1038/s41598-017-06471-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Altered expression of miR-155 and SHIP1 was implicated in the anti-inflammatory and retinal protective activities of Rb1 and Rd. A. RAW264.7 cells were first treated with Rb1 or Rd at the indicated concentrations for 30 min, followed by LPS stimulation delivered at 5 ng/ml for 6 h. Total RNA was isolated from harvested cells and real-time PCR analyses were performed to examine the expression of miR-155 (A) and its target gene SHIP1 (B) (n = 4–6 per group). Relative fold change was calculated against that detected in vehicle-treated cells without LPS stimulation. The data were expressed as the mean ± S.E.M. *Compared to that from vehicle-treated cells without LPS stimulation, p < 0.05; ^#compared to that from vehicle-treated cell with LPS stimulation, p < 0.05. Dark-adapted BALB/c mice were treated with saline vehicle (Vehicle) or combination of Rb1 (65 mg/kg bw) and Rd (22.5 mg/kg bw) (Rb + Rd), followed by bright light exposure. Retinas were collected 6 h and 1 d after illumination along with those from vehicle-treated mice unexposed to bright light (No light). Total RNA was extracted and reverse-transcribed. Real-time PCR was subsequently performed to analyze the retinal expression of miR-155 (C) and SHIP1 (D) (n = 4–6 per group). Relative fold change of miR-155 and SHIP1 was calculated against that detected in vehicle-treated mice unexposed to bright light. The data were expressed as the mean ± S.E.M. *Compared to that from No light, p < 0.05; ^#compared to that from Vehicle, p < 0.05.