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. 2017 Jul 20;7:6015. doi: 10.1038/s41598-017-06471-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Rb1 or Rd treatment suppressed LPS-stimulated expression of proinflammatory genes in ARPE19 cells. Rb1 or Rd was applied to ARPE19 cells at the indicated concentrations 30 min before LPS stimulation delivered at 1 μg/ml for 6 h. Total RNA was extracted for analyzing the expression of Ccl2, IL-1β and IL-6 by real-time PCR (n = 4 per group). The data were expressed as the mean ± S.E.M. *Compared to that from vehicle-treated cells without LPS, p < 0.05; ^#compared to that from vehicle-treated cell with LPS stimulation, p < 0.05.