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. 2017 Jul 20;7:6018. doi: 10.1038/s41598-017-06073-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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GCaMP2 is expressed in astrocytes and interneurons (A) Astrocytic and neuronal localization of GCaMP2 protein in organotypic cortical slices as shown by colocalization with the astrocyte-specific GFAP and interneuron-specific GAD-67 markers. Cells showing colocalization are marked by arrows. Scale bars: 50 μm. (B) Changes in GCaMP2 and GFAP expression as measured by average intensity within cell ROIs (n = 22–34 cells) in organotypic cortical slices as a function of days in culture (DIV). (C,D) Representative traces (top), I/V plot (middle) and action potential generating ability (bottom) of whole-cell patched GCaMP2 expressing astrocytes (C, n = 30) and neurons (D, n = 7) in organotypic cortical slices.