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. 2017 Jul 20;7:6024. doi: 10.1038/s41598-017-06243-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Subcellular localization of HDAC9-EGFP fusion protein in TC organotypic co-culture preparations. (a) Experimental set-up of the co-culture preparation and electroporation into thalamic cells. (b) Schematic representation of the plasmids used in the present study (see the methods). (c) Representative thalamic neurons at 7, 10 and 14 DIV. HDAC9-EGFP distribution in the cells can be compared to the cytoplasmic protein mCherry and the nuclear staining of DAPI. Arrows refer to the position of the same cell at a given time point. Scale bar 10 μm. (d) Quantification of subcellular localization of HDAC9 was expressed as the ratio of transfected cells to illustrate HDAC9-EGFP signal concentrated predominantly in the nucleus (N > C), equally in the nucleus and the cytoplasm (N = C) or predominantly in the cytoplasm (N < C). Statistical analysis by overall Chi-square (Chi = 158, with 6 degrees of freedom, p < 0.000001) followed by pairwise Chi-square with Bonferroni correction. ****p < 0.0001.