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. 2017 Jul 20;7:6024. doi: 10.1038/s41598-017-06243-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Subcellular localization of HDAC9 mutants in thalamic cells and TC axon branching in vitro. Both of nucHDAC9-EGFP (a) and nucHDAC9ΔMEF2-EGFP (b) were distributed in the nuclei of thalamic cells at 14 DIV, while co-expressed mCherry was distributed in cell bodies and dendrites. Green: EGFP signal from the fusion protein. Red: mCherry. Scale bar, 5 μm. (c) Representative mCherry-labeled axon in the cortical explant, which elongated from the transfected thalamic cell. Interrupted line indicates the pial surface of the cortical explant. Scale bar 100 μm.