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. 2017 Jul 20;7:6024. doi: 10.1038/s41598-017-06243-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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HDAC9 nuclear export enhances axon branching in vivo. (a) EYFP-labeled TC axonal projection in the P7 SSp cortex of an animal whose thalamus was electroporated with EYFP plasmid. Coronal section of approximately 60 μm thickness. Scale bar 100 μm. (b) Representative TC axon fragments from control (EYFP-electroporated) and nucHDAC9/mCherry co-electroporated neurons. Coronal sections of 20 μm thickness. Scale bar 50 μm. Statistical analysis by Chi-square (Chi = 22.3, with 3 degrees of freedom), p < 0.0001. (c) Distribution of axon segments according to their branch number. (d) Average branch density. Statistical analysis by Student t-test. *p < 0.05.