Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Jul 20;7:6085. doi: 10.1038/s41598-017-05620-6

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Plasma-activated medium (PAM) inhibits the viability of ovarian cancer cells, depending on the cell type, cell number and PAM dilution ratio. (A) The generation system of PAM and the experimental workflow. (B) H₂O₂ concentrations in each diluted PAM were measured using Amplex red reagent. (C) The sensitivities of ES2 and SKOV3 cells to PAM were analysed according to the LD₅₀ value. (D) Cell viability using the MTS assay at different cell numbers and the corresponding PAM dilution ratio. (E) Morphological changes in ES2 cells after PAM treatment at each dilution ratio. A safe range of PAM with weak cell toxicity is confirmed. (F) After treatment with PAM at the safe range of the dilution ratio for 24 h, ES2 cells were collected for analysis of cell apoptosis markers using western blotting. GAPDH is used as an internal reference. The original full-length blots are presented in Supplementary Figure 3. Data from the MTS assay and H₂O₂ mesurement are presented as mean ± SD. Three replicates were performed.