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. 2017 Jul 20;7:6085. doi: 10.1038/s41598-017-05620-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Plasma-activated medium (PAM) suppresses the migration and invasion abilities of ES2 cells. (A) Wound-healing assay at 0, 24 and 48 h after PAM treatment. (B) Transwell migration and invasion assay. Both migration and invasion abilities are calculated in comparison with the total seeded cells. (C) Proliferation assay for a total of 4 days. On each day, the Aqueous One Solution Cell Proliferation Assay reagent (Promega) is added for indication of cell number. (D) Colony-formation assay. Number of colonies was counted and compared to the total seeded cells. Three representative fields of cells in each group were captured and calculated, while one was selected for presentation in this study. Data are presented as mean ± SD. Three independent experiments with at least three replicates are performed. Statistics are shown as **P < 0.01.