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. 2017 Jul 20;7:6085. doi: 10.1038/s41598-017-05620-6

Figure 4.

Figure 4

Plasma-activated medium (PAM) down-regulates the expression of MMP-9 in ovarian cancer cells. (A) mRNA expressions of MMP-2 and MMP-9 are calculated using real-time quantitative PCR. The MMP-2 expression is not significantly different between each diluted PAM group and the control group (P = 0.554, P = 0.254 and P = 0.533, respectively), while MMP-9 is gradually decreased with PAM (P = 0.839, P < 0.01 and P < 0.01, respectively). (B) Protein levels of MMP-2 and MMP-9 from ES2 cells treated with PAM. GAPDH was used as the internal control. The original full-length blots are presented in Supplementary Figure 3. Data are presented as mean ± SD. Three independent experiments were performed. Each PCR reaction was performed in triplicate. Statistics are shown as *P < 0.05, **P < 0.01.