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. 2017 Jul 20;7:6044. doi: 10.1038/s41598-017-06413-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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BRRF1 is expressed with early kinetics. (a) B95-8 cells were lytically induced by TPA, A23187 and butyrate (T/A/B) with or without PAA¹⁴. (b) AGS-Akata cells were lytically induced by TPA and butyrate (T/B) with or without PAA. Cellular RNA was collected after 48 hours, and qRT-PCR was performed using BALF2, BMRF1, BRRF1, MCP, gp350 and GAPDH primers. Relative mRNA levels for each gene are shown after normalization to GAPDH mRNA levels. Each bar represents the mean and standard deviation of three independent experiments.