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. 2017 Jul 20;7:5956. doi: 10.1038/s41598-017-05798-9

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The mean log10-transformed Bd zoospore load of the Bd-infected A. obstetricans tadpoles is shown for each of the six ponds and the eleven months in the study. The control ponds and the antifungal-treated ponds are in the left and right columns, respectively. The 2010 and 2011 cohorts are shown in white and black, respectively. Fungicide treatment was applied to tadpoles in the months of May, June, July, and September (asterisks). Uninfected tadpoles were not included in the calculation of the mean Bd zoospore load. The bars show the 95% confidence limits of the mean.