Table 2.
IRAP and REMAP primer combinations used in this study.
| Primer | Annealing temp. (°C) | Loci | He | Ne | I | Band size (bp) | ||
|---|---|---|---|---|---|---|---|---|
| IRAP | Total | Polymorphic | % polymorphic | |||||
| Sukkula LTR1 | 59 | 15 | 12 | 80 | 0.36 | 1.44 | 0.38 | 75–1500 |
| Wilma-Bagy2 | 60 | 19 | 19 | 100 | 0.31 | 1.48 | 0.41 | 300–2000 |
| Daniela LTR2 | 60 | 20 | 12 | 60 | 0.24 | 1.74 | 0.55 | 400–1500 |
| Fatima LTR | 60 | 14 | 13 | 92.8 | 0.30 | 1.57 | 0.41 | 300–2000 |
| 5LTR-Sukkula LTR1 | 61 | 16 | 15 | 93.7 | 0.33 | 1.65 | 0.48 | 200–1300 |
| 5LTR-Daniela LTR1 | 61 | 12 | 12 | 100 | 0.35 | 1.43 | 0.47 | 200–700 |
| Bare1 LTR-Sukkula LTR1 | 60 | 10 | 10 | 100 | 0.23 | 1.43 | 0.47 | 200–1500 |
| Bare1 LTR-Daniela LTR2 | 60 | 13 | 12 | 92.3 | 0.28 | 1.55 | 0.33 | 100–1500 |
| Bare1 LTR-Sukkula LTR2 | 63 | 18 | 16 | 88.8 | 0.34 | 1.63 | 0.47 | 200–2000 |
| Tms1Ret1 | 63 | 12 | 9 | 75 | 0.30 | 1.45 | 0.41 | 100–1500 |
| LORE1 | 58 | 11 | 8 | 72.7 | 0.29 | 1.33 | 0.42 | 200–1500 |
| LORE2 | 55 | 9 | 6 | 66.7 | 0.24 | 1.54 | 0.48 | 200–1500 |
| Tps12a | 54 | 4 | 1 | 25 | 0.23 | 1.44 | 0.38 | 200–700 |
| Tms1Ret1- LORE1 | 61 | 5 | 3 | 60 | 0.22 | 1.23 | 0.45 | 200–1000 |
| Tms1Ret1- LORE2 | 60 | 12 | 10 | 83.3 | 0.30 | 1.32 | 0.55 | 300–1500 |
| Tms1Ret1- Tps12a | 58 | 13 | 8 | 61.5 | 0.32 | 1.35 | 0.44 | 300–1500 |
| LORE1-LORE2 | 58 | 9 | 6 | 66.7 | 0.25 | 1.38 | 0.46 | 300–1500 |
| LORE1-Tps12a | 59 | 6 | 4 | 66.7 | 0.24 | 1.37 | 0.42 | 420–1500 |
| LORE2-Tps12a | 56 | 7 | 1 | 14.2 | 0.20 | 1.34 | 0.38 | 800–15000 |
| REMAP | ||||||||
| WIS2–1A LTR-UBC815 | 63 | 12 | 12 | 100 | 0.41 | 1.70 | 0.59 | 400–1500 |
| Sukkula LTR1-UBC815 | 57 | 15 | 13 | 86.7 | 0.35 | 1.69 | 0.54 | 100–1500 |
| Wilma-Bagy2-UBC815 | 63 | 18 | 15 | 83.3 | 0.39 | 1.74 | 0.62 | 300–1500 |
| Daniela LTR1-UBC815 | 58 | 12 | 10 | 83.3 | 0.43 | 1.32 | 0.54 | 200–2000 |
| Daniela LTR2-UBC815 | 62 | 11 | 10 | 90.9 | 0.38 | 1.50 | 0.26 | 500–2000 |
| Fatima LTR-UBC815 | 58 | 18 | 16 | 88.8 | 0.45 | 1.33 | 0.36 | 300–2000 |
| Wis LTR2-UBC818 | 58 | 17 | 12 | 70.5 | 0.21 | 1.47 | 0.48 | 75–1500 |
| Sukkula LTR1-UBC825 | 60 | 16 | 14 | 87.5 | 0.30 | 1.25 | 0.62 | 200–2000 |
| Wilma-Bagy2-UBC840 | 60 | 12 | 11 | 91.7 | 0.40 | 1.58 | 0.58 | 300–2000 |
| Daniela LTR1-UBC840 | 60 | 15 | 13 | 86.7 | 0.38 | 1.65 | 0.45 | 200–2000 |
| Wis LTR2-UBC848 | 57 | 16 | 15 | 93.7 | 0.32 | 1.50 | 0.53 | 300–1500 |
| WIS2–1A LTR-UBC857 | 58 | 14 | 14 | 100 | 0.40 | 1.69 | 0.62 | 200–2000 |
| Sukkula LTR1-UBC857 | 60 | 12 | 12 | 100 | 0.39 | 1.48 | 0.64 | 100–1500 |
| Wis LTR1-UBC857 | 58.9 | 13 | 11 | 84.6 | 0.34 | 1.47 | 0.58 | 300–1500 |
| Wis LTR2-UBC857 | 59.4 | 10 | 9 | 90 | 0.32 | 1.54 | 0.62 | 300–1500 |
| Daniela LTR1-UBC857 | 60.7 | 12 | 5 | 41.6 | 0.21 | 1.35 | 0.54 | 200–1500 |
| Daniela LTR2-UBC857 | 58.3 | 11 | 8 | 72.7 | 0.25 | 1.44 | 0.58 | 200–1500 |
| Wis LTR1-A13 | 58 | 18 | 17 | 94.4 | 0.36 | 1.65 | 0.49 | 300–1500 |
| Wilma-Bagy2-A13 | 62 | 15 | 15 | 100 | 0.38 | 1.80 | 0.53 | 200–1500 |
| Daniela LTR1-A13 | 62 | 11 | 11 | 100 | 0.39 | 1.78 | 0.53 | 300–1500 |
| Tms1Ret1–459 | 52 | 14 | 11 | 78.5 | 0.44 | 1.33 | 0.48 | 500–2500 |
| Tms1Ret1-A7 | 52 | 5 | 1 | 20 | 0.38 | 1.45 | 0.34 | 350–850 |
| Tms1Ret1-A12 | 52 | 12 | 6 | 50 | 0.35 | 1.41 | 0.47 | 700 |
| Tms1Ret1-B1 | 52 | 2 | 1 | 50 | 0.34 | 1.32 | 0.38 | 200–1000 |
| Tms1Ret1–438 | 51 | 8 | 5 | 62.5 | 0.41 | 1.23 | 0.39 | 500–1000 |
| Tms1Ret1–443 | 52 | 5 | 2 | 40 | 0.38 | 1.48 | 0.42 | 150–700 |
| Tms1Ret1–818 | 52 | 6 | 3 | 50 | 0.39 | 1.55 | 0.41 | 200–1000 |
| Tms1Ret1–825 | 52 | 9 | 4 | 44.4 | 0.41 | 1.35 | 0.42 | 200–850 |
| LORE1–840 | 52 | 8 | 2 | 25 | 0.36 | 1.74 | 0.52 | 200–1000 |
| LORE1–848 | 52 | 7 | 3 | 42.8 | 0.38 | 1.65 | 0.51 | 200–1400 |
| LORE2–849 | 52 | 9 | 3 | 33.3 | 0.42 | 1.55 | 0.42 | 350–1000 |
| LORE2–857 | 52 | 12 | 5 | 41.6 | 0.32 | 1.30 | 1.38 | 500–1000 |
| Tps12a-459 | 52 | 9 | 7 | 77.7 | 0.33 | 1.25 | 1.23 | 300–1000 |
| Tps12a-438 | 52 | 7 | 4 | 57.1 | 0.40 | 1.45 | 0.35 | 200–1500 |