Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Jul 20;7:6020. doi: 10.1038/s41598-017-06135-w

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

pH dependence of the second zinc binding. ¹H, ¹³C HSQC spectra encompassing the aromatic peak region were acquired for one- and two-zinc LytU with ~0.4 pH unit intervals. Only titration figures at the lower end of the range (4.0–6.2) are provided due to the lack of differences at higher pH values. Shown are the overlays of the histidine ε1 region of one- and two-zinc LytU spectra at different pHs. One-zinc LytU is represented with blue contours and two-zinc LytU with red contours. At low pH, the C^ε1-H^ε1 regions of the ¹H, ¹³C HSQC spectra of one- and two-zinc LytU are identical: H76 and H159 ε1 peaks have same positions and no peaks are observed for H125 or H157. When pH is raised, H76 and H159 ε1 peaks at positions corresponding to the two-zinc form appear and gain intensity. In addition, H125 and H157 ε1 peaks emerge. At pH 5.2 the one- and two-zinc His ε1 peaks have approximately equal intensity, and at pH over 6.5 one-zinc LytU disappears from the two-zinc LytU sample.