Figure 2.

Proteomics of feverfew pollen reveals the presence of allergenic protein families. (a) Summarized workflow and results of the proteomic analyses. (b) Two-dimensional gel electrophoresis of feverfew pollen extract. After the isoelectric focusing, proteins were separated by SDS-PAGE and the spots were visualized by Coomassie staining. All excised spots subjected to mass analysis are circled and spots containing allergenic molecules are indicated in red circles. Detailed information of identified allergenic protein families is shown in Table 1. The unprocessed gel is shown in the Related Manuscript File.