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. 2017 Jul 20;7:6049. doi: 10.1038/s41598-017-06213-z

Figure 3.

Figure 3

Immunoblot analysis of feverfew pollen extract (E) and purified recombinant Par h 1 (P). Extract and Par h 1 were loaded on reducing SDS-PAGE and stained with Coomassie. Immunoblot analysis was performed with a serum pool of Austrian weed pollen allergic patients (n = 15) and Indian feverfew pollen sensitized patients (n = 25). The unprocessed gels and immunoblots are shown in the Related Manuscript File. NHS, non-atopic human sera (n = 2) and BC, buffer control.