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. 2017 Jul 20;7:6089. doi: 10.1038/s41598-017-06470-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Mouse epithelial progenitor cells expanded by TGF-β inhibition are capable of differentiation in vitro. (a–d) Newborn mouse-derived epithelial progenitor cells expanded by RepSox treatment were grown in CnT-PR media in the presence of 1 μM RepSox to sub-confluence, followed by cultivation in cFAD for 7 days in the absence of RepSox. Epithelial progenitor cells used were derived from the (a) esophagus, (b) salivary gland, (c) bladder, and (d) thymus. Bar graphs on the left, quantitative RT-PCR analysis of epithelial cell differentiation marker genes, p63, CK4, CK7, CK18, Ivl, and Aqp5. Data shown are normalized to the housekeeping gene Gapdh and expressed as mean ± s.e.m. (n = 3). *P < 0.05; **P < 0.01. Right, Western blot analysis with antibodies against p63, CK4, CK7, CK18, aquaporin 5 (AQP5), and involucrin (IVL). Tubulin-α (Tub) was used as a loading control. The full-length blots are included in Supplementary Figure S13.