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Mössbauer spectroscopy provides evidence for the presence of an [Fe(III)‐Fe(IV)] species during catalysis of Fe(II) oxidation in subunits whose sites A, B, and C are occupied with Fe(II). Mössbauer spectrum was recorded for a sample quenched circa 1.0 s after aerobic addition of 48 57Fe(II) per ferritin 24‐mer (45 μm) to apo‐PfFtn. The black solid line (Sum) is the superposition of the simulated spectra. Measurements were performed at 80 K.
