Table 1.
Iron species observed in PfFtn at different times points for addition of 48 Fe(II) per ferritin 24‐mer
| Time (s) | Doublet | Fe species | % of 57Fe(II) | Mössbauer parameters | Ref. | |
|---|---|---|---|---|---|---|
| δ (mm/s) | ΔEQ (mm/s) | |||||
| 0 | 1 | Fe(II) | 19(1) | 1.39 (1) | 3.27 (1) | 10 |
| 2 | Fe(II) | 41(2) | 1.38 (1) | 2.73 (2) | ||
| 3 | Fe(II) | 40(1) | 1.17 (1) | 2.54 (1) | ||
| 0.7 | 1 | Fe(II) | 16(2) | 1.20 (1) | 2.77 (1) | 10 |
| 2 | Fe(III)a | 42(1) | 0.49 (1) | 0.76 (1) | ||
| 3 | Fe(III)b | 42(1) | 0.56 (2) | 1.12 (1) | ||
| 1 | 1 | Fe(II) | 8(1) | 1.20 (1) | 2.77 (2) | This work |
| 2 | Fe(III)a | 39(1) | 0.46 (2) | 0.76 (1) | ||
| 3 | Fe(III)b | 39(1) | 0.56 (1) | 1.12 (1) | ||
| 4 | Fe(III) | 9(2) | 0.69 (1) | 0.97 (2) | ||
| 5 | Fe(IV) | 5(2) | 0.25 (2) | 0.46 (2) | ||
| 300 | 1 | Fe(III) | 42 | 0.49 (1) | 1.14 (1) | 10 |
| 2 | Fe(III) | 58 | 0.48 (1) | 0.67 (1) | ||
At time 0 no dioxygen was added. After the addition of dioxygen samples were rapidly frozen at 0.7, 1, and 300 s.
a,bThe Fe(III) doublets were assigned to the peroxodiferric intermediate in the ferroxidase center.