Figure 2.

F13 lacking palmitoylation sites fails to associate with KLC. HEK293T cells were co‐transfected with plasmids expressing FLAG‐KLC1 and HA‐F13co, or HA‐F13coC185186S, or HA‐GFP. Cell lysates were harvested 36 h post‐transfection and HA‐tagged proteins were immunoprecipitated and analysed by SDS‐PAGE and immunoblotting with the indicated antibodies. Cell lysates prior to immunoprecipitation (Input) were run in parallel. The immunoblots shown are representative of multiple experiments (n = 3).