Figure 3.

Example results in primary ciliary dyskinesia and “primary ciliary dyskinesia highly unlikely” (control) samples for three antibodies used in the immunofluorescence panel. The left column shows the cell nucleus in blue by 4’,6-diamidino-2-phenylindole (DAPI); the next column shows presence of cilia on the cell in green by acetylated tubulin. The third column shows the protein of interest in red, and the final column a merged image of the three channels. In the merged image yellow suggests colocalization and presence of the protein of interest, and green suggests absence of the protein of interest. The top images show DNALI1 (an inner dynein arm component), the middle images DNAH5 (an outer dynein arm component), and the bottom images RSPH4A (a radial spoke head component).