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. 2017 Jul 14;8:16085. doi: 10.1038/ncomms16085

Table 1. Primer list.

Primer Primer sequence 5′3′ Application
16S Fw ATGACGGCCTTCGGGTTGTAA qRT–PCR
16S Rv CGGCTGCTGGCACGTAGTTG qRT–PCR
ESAT6 Fw AGGGTGTCCAGCAAAAATGG qRT–PCR
ESAT6 Rv CTGCAGCGCGTTGTTCAG qRT–PCR
CFP10 Fw GCAGGAGGCAGGTAATTTCG qRT–PCR
CFP10 Rv CCTGGTCGATCTGGGTTTTC qRT–PCR
Ag85A Fw ATGCAGCTTGTTGACAGGGTT qRT–PCR
Ag85A Rv TCGACGCGACATACCCGT qRT–PCR
Ag85B Fw GGTTCAGTTCCAGAGCGGTG qRT–PCR
Ag85B Rv TCGAGCAGATAAACCGCAGG qRT–PCR
EspA Fw GGCACCCTCGGAGAAGTGT qRT–PCR
EspA Rv AGCTCTTTCAGGCCGTTGAG qRT–PCR
EspC Fw TGTACTTGACTGCCCACAATGC qRT–PCR
EspC Rv TCGACACCGGCCGTATG qRT–PCR
E6C10 P1 ACATTTTGGCGAGGAAGGTAAAGAGAGAAAGTAGTCCAGCGTGTAGGCTGGAGCTGCTTC MTBVACΔE6C10
E6C10 P2 GATCCCGTGTTTCGCTATTCTACGCGAACTCGGCGTTGCCCATATGAATATCCTCCTTAGT MTBVACΔE6C10
E6C10 Fw TCCCGTAATGACAACAGACTTC MTBVACΔE6C10
E6C10 Rv GGAAGAGCTTGTCGTAGTCG MTBVACΔE6C10
E6C10 P1A AGCGGATTTGACGTCGTGCT MTBVACΔE6C10
E6C10 P1B CGATTGTCGCCCTACCCGAT MTBVACΔE6C10
Ag85B P1 CCCGAGCACACGACGACATACAGGACAAAGGGGCACAAGTGTGTAGGCTGGAGCTGCTTC MTBVACΔAg85B
Ag85B P2 ATCTCACGTGGACGGGTAAGCAACCCTTCGGTTGATCCCGCATATGAATATCCTCCTTAGT MTBVACΔAg85B
Ag85B Fw ACATTTGGCCTCCACACAC MTBVACΔAg85B
Ag85B Rv CAATCAGCGACAACAGGATGCC MTBVACΔAg85B
Ag85B P1A TAGCACTCGAGTGATCGGCT MTBVACΔAg85B
Ag85B P1B CCTCGAACCAACCGCCTTC MTBVACΔAg85B