Table 1. The effect of M. aeruginosa allelopathy on the proteome of C. vulgaris.
| Up or down expression | Protein names and categories | Ratio | P-value |
|---|---|---|---|
| Photosynthesis | |||
| ▵ | Cytochrome b6-f complex subunit 4 | 0.7 | 3.44E−03 |
| ▾ | Photosystem I iron-sulfur center | 0.4 | 6.82E−09 |
| ▾ | Light-independent protochlorophyllidereductase subunit N | 0.7 | 2.04E−02 |
| ▾ | Photosystem II protein D1 | 0.5 | 6.51 E−04 |
| ▾ | Photosystem II reaction center protein H | 0.7 | 1.04E−04 |
| ▾ | Photosystem I reaction center subunit IX | 0.6 | 2.4 E−02 |
| ▾ | Photosystem I P700 chlorophyll a apoprotein A | 0.2 | 2.28E−04 |
| ▾ | Ribulose bisphosphate carboxylase large chain | 0.3 | 1.50E−02 |
| ▾ | Ribulose bisphosphate carboxylase small chain | 0.4 | 2.27E−03 |
| ▾ | Ferredoxin—NADP reductase | 0.7 | 7.88E−05 |
| ▵ | Cytochrome f | 1.3 | 2.97E−05 |
| Membrane transporters | |||
| ▾ | 1-acyl-sn-glycerol-3-phosphate acyltransferase | 0.5 | 4.23E−02 |
| ▵ | ‘Cytochrome b–c1 complex subunit Rieske, mitochondrial’ | 1.4 | 3.80E−02 |
| Protein homeostasis | |||
| ▾ | ‘30S ribosomal protein S8, chloroplastic’ | 0.3 | 3.88E−02 |
| ▵ | 40S ribosomal protein S4 | 1.4 | 2.24E−07 |
| ▵ | 40S ribosomal protein S6 | 1.4 | 5.95E−03 |
| ▵ | Ribosomal protein S7 | 1.4 | 6.56E−03 |
| ▵ | 40S ribosomal protein S27 | 1.5 | 7.57E−03 |
| ▵ | 40S ribosomal protein S8 | 1.4 | 3.03E−03 |
| ▵ | 60S ribosomal protein L13 | 1.4 | 1.97E−05 |
| ▵ | 40S ribosomal protein S18 | 1.6 | 4.41E−05 |
| ▵ | Peptidyl-prolylcis-trans isomerase (fragment) | 1.5 | 1.06 E−02 |
| Energy | |||
| ▾ | Kinesin-like protein | 0.7 | 3.47E−03 |
| ▵ | Glucose-6-phosphate 1-dehydrogenase | 1.5 | 2.18E−02 |
| ▵ | ATP synthase F0 subunit beta | 1.6 | 1.25E−04 |
| ▵ | Glyceraldehyde-3-phosphate dehydrogenase (fragment) | 1.3 | 2.14E−02 |
| ▵ | Fructose-bisphosphate aldolase | 1.7 | 4.92E−04 |
| ▵ | Glycerol-3-phosphate dehydrogenase [NAD(+)] | 1.4 | 1.21E−03 |
| The uptake and transportation of nitrogen | |||
| ▾ | Nitrate transporter NTR | 0.4 | 2.90E−02 |
| ▵ | Nitrate reductase | 1.6 | 4.77E−02 |
| ▵ | Ferredoxin-nitrite reductase | 1.4 | 2.85E−03 |
| Stress and defense | |||
| ▵ | Glutaredoxin | 1.4 | 8.78 E−06 |
| ▵ | Cysteine synthase | 1.6 | 2.24 E−03 |
| Signal transduction | |||
| ▵ | Putative nitric oxide synthase | 1.5 | 4.47E−02 |
| Stability and repair of DNA structure | |||
| ▾ | Histone H2B | 0.4 | 3.29E−02 |
| ▾ | Histone H4 | 0.5 | 8.67E−05 |
| ▾ | Heat shock protein 90 | 0.6 | 6.41E−03 |
| ▾ | GrpE protein homolog (fragment) | 0.5 | 1.04E−02 |
| Nucleoside metabolic process | |||
| ▵ | Adenylosuccinatelyase | 1.5 | 9.78 E−07 |
| Fatty-acid metabolism | |||
| ▵ | Acyl-coenzyme A synthase | 1.4 | 2.65 E−07 |
| ▵ | 3-Oxoacyl-[acyl-carrier-protein] synthase | 1.3 | 3.31 E−03 |
Differentially expressed proteins that were significantly over (▵) and under (▾) expressed by >1.2 and <0.83, respectively, in C. vulgaris exposed to M. aeruginosa allelopathy relative to protein expression in C. vulgaris monocultures are shown and categorized in metabolic pathways and cellular functions.