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. 2017 Jul 17;8:16069. doi: 10.1038/ncomms16069

Figure 5. Expansion of H3K27ac marks upon deletion of CTCF sites.

Figure 5

ChIP-seq was used to identify changes of H3K27ac due to mutated CTCF-binding sites. (a) Deletion of CTCF site C resulted in the expansion of H3K27ac associated with the Wap super-enhancer (red bar). This was not observed upon loss of sites D or E. The combined loss of sites C, D and E resulted also in an expansion of H3K27ac and was particular strong at a specific site in the first intron of Ramp3 (normalized to 10 million reads) (red bar). (b) H3K27ac intensity was equivalent in the unrelated Bcl6 locus. (c) H3K27ac profiles of the Wap-Ramp3 locus based on the data shown in a. Green, WT pattern; red, ΔC. The degree of H3K27ac in mutants was elevated between CTCF sites C and E confirming the expansion of H3K27ac (normalized to 10 million reads). (d) H3K27ac profiles of the data shown in a. WT is shown in green, ΔCDE in red. H3K27ac marks spread from site E to the second exon of Ramp3 (normalized to 10 million reads).