Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Jul 17;8:16064. doi: 10.1038/ncomms16064

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2017, The Author(s)

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

(a) Procedure of the single-molecule total internal reflection fluorescence (TIRF) microscope experiment. (b) Negative staining EM micrograph of the ESCRT-III filaments. Scale bar, 50 nm. (c) TIRF microscopy images of Cy3 fluorescence channel representing ESCRT-III filaments after immobilization (left), after disassembly by Vps4 (middle) and after disassembly by Vps4 and Vta1 (right). Scale bar, 10 μm. (d) The average Cy3 fluorescence intensities associated with filaments under various conditions of the disassembly reaction. Data are represented as the average of three independent experiments. Error bars represent s.e.m. (e,f) Single-molecule fluorescence trajectories of Cy5-Vps4 and Cy3-filament during the filament disassembly. (e) Cy5-Vps4 disappeared while almost no changes were observed in the filament coupled Cy3 fluorescence intensity. (f) Cy5-Vps4 disappeared while the filament coupled Cy3 fluorescence intensity was also decreased.