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. 2017 Mar 17;11(7):1592–1601. doi: 10.1038/ismej.2017.31

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Copyright © 2017 The Author(s)

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Growth of R. pomeroyi DSS-3 wild-type (WT), and the dmmD mutant (dmmD::Gm) complemented with either the four-gene cluster dmmDABC (dmmD::Gm+dmmDABC) or only the structural genes dmmABC (dmmD::Gm+dmmABC) along with the promoter on different nitrogen sources. Nitrogen was supplied in the form of (a) TMA, (b) TMAO, (c) DMA and (d) MMA. Solid lines represent cell growth. Dashed lines represent the degradation of the appropriate substrate with the concentrations of TMA, TMAO, DMA and MMA being quantified throughout the whole experiment. Results presented are the mean of triplicates and error bars denote s.d.