Figure 2.

miR-30a impairs growth and tumorigenicity of KRAS-mutant CRC cells. (a) miR-30a-5p/3p levels were measured in CRC cells with miR-30a overexpression or KO. (b–d) miR-30a overexpression inhibits cell viability (b), focus formation (c) and anchorage-independent soft agar growth (d) in HCT116, DLD1 KRAS-mutant CRC cells. MiR-30a KO showed opposite effect in KRAS-mutant CRC cells. Scale bar, 200 μm. Colonies of focus formation and anchorage-independent soft agar were counted and showed in bar graphs. (e) Overexpression of miR-30a promotes apoptosis of KRAS-mutant CRC cells as indicated by immunoblot using PARP-1 antibody. β-actin was used as the loading control. (f) miR-30a selectively slows tumor growth in HCT116 and DLD1 xenograft (n=5). Data are shown as the means±S.D. *P<0.05, **P<0.01, ***P<0.001