Figure 4.

ME1 is upregulated in human KRAS-mutant CRCs. (a) Left: expression of ME1 normalized to β-actin was measured by RT-qPCR in paired colorectal tissues. Right: data from CRC tissues were showed as WT versus KRAS-mutant group (Mut) according to KRAS status. (b) ME1 is upregulated in CRC tissues from TCGA database. Left: paired tissues; Right: unpaired tissues. (c) Protein levels of ME1, KRAS and P65 were detected by immunoblot in colorectal tissues. β-actin was used as the loading control. (d) ME1, ME2 mRNA expression in KRAS WT and Mut cancer cells from CCLE database. (e) Protein levels of ME1, KRAS and P65 in CRC tissues. β-actin was used as the loading control. N, normal tissues; T, CRC tissues