Figure 6.

miR-30a overexpression and ME1 suppression attenuate AOM-/DSS-induced colorectal tumorigenesis in mice. (a) Treatment schedule with lentivirus containing miR-30a expression vector during AOM-/DSS-induced colorectal tumorigenesis in mice. Indicated lentivirus was intrarectally administrated into mice (n=10 per group) weekly for four times. Mice were killed 100 days after AOM injection. (b) Relative miR-30a-5p/3p levels were detected in colorectal tissues by RT-qPCR from (a). (c) Protein levels of KRAS, P65 and ME1 were detected in colorectal tissues by immunoblot. β-actin was used as the loading control. (d) ME1 and ME2 activity of colorectal tissues from (a). (e) Representative images of mouse colorectal tumors were showed. Immunohistochemistry of mouse colorectal tumors of miR-30a overexpression, KRAS and ME1 suppression showed decreased Ki-67 and increased cleaved caspase-3 staining compared with the control group. (f) The numbers of tumors in the mouse colorectum were counted and percentages of tumors of <2 mm or 2–4 mm in diameter were quantified. Data are shown as the means ± S.D. *P<0.05, **P<0.01, ***P<0.001