Figure 3.

Psoralidin-induced apoptosis in CTPE cells. CTPE cells were treated with 4 μM Pso for 12 and 24 h and the levels of Bax, cleaved caspase-9, caspase-3 and PARP were measured by western blotting. Densitometric analysis of western blots from one, two or three experiments are given in Supplementary Information. β-actin was used as a loading control. CTPE cells were treated either with vehicle or Pso for 24 h and fixed with paraformaldehyde and permeabilised with sodium citrate and Triton X. DNA fragmentation was determined by TdT-mediated dUTP nick end labelling (TUNEL) (red channel). DAPI (blue channel) was used to locate the nuclei of the cell and numbers of cells were counted manually. Student’s t-test was used to calculate statistical significance between vehicle control and Pso treatment. **P<0.01