Figure 2. Up-regulated miR-143 decreased expression and phosphorylation of ERK5and MAP3K7 in MCF-7 cells. Cells were transfected with mimic control, miR-143 mimic, inhibitor control or miR-143 inhibitor. A, miR-143 levels and mRNA levels of ERK5 and MAP3K7 using RT-qPCR. B, Expression and phosphorylation levels of ERK5 and MAP3K7 using western blot analysis. ERK5: extracellular signal-regulated kinase 5; MAP3K7: mitogen-activated protein 3 kinase 7. Data are reported as means±SD of three independent experiments. **P<0.01 compared with control (ANOVA).
