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. 2017 Jun 7;6(6):e49. doi: 10.1038/emi.2017.35

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Copyright © 2017 The Author(s)

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Study design. nasopharyngeal aspirate, NPA; quantitative PCR with reverse transcription, qRT-PCR. ^aRoutine clinical testing was performed using antigen detection by the DFA, which included the influenza A and B viruses, parainfluenza virus types 1–3, respiratory syncytial virus, human metapneumovirus and adenovirus. From 1 March to 8 April 2015 (during the peak influenza A virus season), monoplex real-time RT-PCR for the influenza A M gene was performed for patients admitted to the general medical ward. ^bPatients whose NPA specimens either tested negative for respiratory viruses by DFA or had insufficient NPCs for DFA during routine clinical testing. Insufficient NPCs is defined as <20 NPCs in the entire well.