Table 1. Primers and probes used in the quantitative reverse transcription PCR.

Virus	Primer/probe sequence (5′-3′)
Influenza A virus	Forward	GACCRATCCTGTCACCTCTGAC
	Reverse	AGGGCATTYTGGACAAAKCGTCTA
	Probe	FAM- TGCAGTCCTCGCTCACTGGGCACG -BHQ1
Influenza B virus	Forward	ACAATTGCCTACYTGCTTTCA
	Reverse	TCTTTCCCACCRAACCAAC
	Probe	HEX- AGAAGATGGAGAARGCAAAGCAGAACTAGC -IABkFQ
Human metapneumovirus	Forward	CATAYAARCATGCTATATTRAAAGAGTCTC
	Reverse	CCTATYTCWGCAGCATATTTGTAATCAG
	Probe	FAM- CAACHGCAGTRACACCYTCATCATTRCA -IABkFQ
Respiratory syncytial virus	Forward	CTTAGCAAAGTCAAGTTRAATGATACA
	Reverse	TGCACATCATAATTRGGAGTGTC
	Probe	HEX- ACYATYCAACGKAGYACAGGAGA -IABkFQ
Parainfluenza virus type 1	Forward	GGAGGAGCAATTATACCTGGTCA
	Reverse	TGTATCCARTGAGTGGGCTA
	Probe	LC610- ATTAGGCCCGAGTGTRACRGATGATGC -BBQ
Parainfluenza virus type 2	Forward	TATGCYATGGTGGGAGACATT
	Reverse	GCCATCTTGTTCCAAGTCCAT
	Probe	FAM- CCTCCCATTCCGCTGTGTTCAATRTACTT -IABkFQ
Parainfluenza virus type 3	Forward	AGCTATYACTAGYATCTCAGGGT
	Reverse	CCCAATCTGATCCACTGTGT
	Probe	HEX- TCAGACAAGATGGAACAGTGCAGGCA -IABkFQ