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. 2017 Jun 21;6(6):e55. doi: 10.1038/emi.2017.41

Figure 1.

Figure 1

The evaluation of the T5 exonuclease digestion. In each case, 1 μg of the pAM6 plasmid (7.5 kb) was used. The linearization of the plasmid was performed with 10 units of SpeI enzyme (NEB, Ipswich, MA, USA) at 37 °C for 1 h. The T5 exonuclease digestion was performed with 10 units of the enzyme (Epicentre) at 37 °C for 30 min. The positions of the linear and covalently closed circular (ccc) plasmid forms are indicated. The electrophoresis was carried out in 0.8% agarose gel in 1 × TAE buffer. M indicates the DNA marker.