Table 3.
Descriptive statistics of novel Passiflora edulis microsatellite markers
| # | Marker | Primer Sequences 5′-3′ | RepeatMotif | Ta | AlleleN | Allele Size Range (bp) |
He | Ho | PIC |
|---|---|---|---|---|---|---|---|---|---|
| 1 | BrPe0032 | F:TTGCACAATGACCAATGTTGT R:CTGAGCACCTTGTCAAAATACA |
(AT)13 | 60 °C | 7 | 137–157 | 0.84 | 1.00 | 0.77 |
| 2 | BrPe0028 | F:CAAAAGGAACAGGGAAGA R:GAAAGAGAGAAAGACAGAGA |
(TA)6 | 55 °C | 5 | 90–110 | 0.80 | 0.50 | 0.72 |
| 3 | BrPe0024 | F:CCCTACCTTTCTCTGCTT R:CATCTCCTCTATCTCCTTC |
(TC)7 | 55 °C | 4 | 221–231 | 0.76 | 0.70 | 0.68 |
| 4 | BrPe0031 | F:AGGTCGGTGGGTGTGTTTAG R:CATTCAACTCCCCAAAAGGT |
(TA)9 | 60 °C | 5 | 134–150 | 0.77 | 0.67 | 0.67 |
| 5 | BrPe0014 | F:AATATGGCTGGGGAAAAC R:TTCCTGTCTTTGGACCTT |
(AG)7 | 57 °C | 5 | 215–227 | 0.75 | 0.50 | 0.67 |
| 6 | BrPe0033 | F:GCCATGAGAGACTTGGGAGA R:CGGTTGCCAAAAAGAAGAGA |
(AT)8 | 60 °C | 5 | 237–249 | 0.72 | 0.30 | 0.65 |
| 7 | BrPe0038 | F:TTTCAACTTTTCGTGTGTGC R:TGTTGTTGCTTGGAAGGATG |
(AT)6 | 60 °C | 5 | 154–176 | 0.73 | 0.60 | 0.64 |
| 8 | BrPe0042 | F:CATGCATTCATTTGTTTTTCTTG R:GATGCTGGGAAAAAGAGTGC |
(AT)8 | 60 °C | 6 | 142–160 | 0.71 | 0.80 | 0.63 |
| 9 | BrPe0003 | F:CTTTCTCTCCCTATACCC R:CCCTCCATAATCACATAAC |
(TC)11 | 55 °C | 5 | 277–291 | 0.70 | 0.40 | 0.62 |
| 10 | BrPe0043 | F:TCATACATGGATGTCAAATCGATAC R:GCGGACCAAGAAAATTCAAA |
(AT)8 | 60 °C | 4 | 199–207 | 0.71 | 0.50 | 0.60 |
| 11 | BrPe0006 | F:AAGGAAAAGAACAGCCTCA R:CGCTCTCAAATCAGTCAAA |
(TC)10 | 55 °C | 4 | 193–199 | 0.68 | 0.40 | 0.59 |
| 12 | BrPe0002 | F:AAAGCCCAGATGAAGTGAA R:GGCTCCAATCAGAAGTGT |
(AG)12 | 55 °C | 3 | 177–185 | 0.69 | 0.56 | 0.58 |
| 13 | BrPe0021 | F:ACTTCCTCATCATTCG R:GCTATGCCTCTTTTTG |
(TA)7 | 55 °C | 3 | 158–164 | 0.67 | 0.25 | 0.56 |
| 14 | BrPe0036 | F:TCGGACCTTAAAACCGAGAA R:CAGCACCAAAATTTGACGAG |
(TC)6 | 60 °C | 4 | 197–203 | 0.65 | 0.10 | 0.54 |
| 15 | BrPe0023 | F:AGATACCACACCCAATAG R:TTGGAGTTGTTGGGGA |
(CT)7 | 55 °C | 6 | 118–132 | 0.57 | 0.40 | 0.52 |
| 16 | BrPe3011 | F:CCGGTCTTCCTGATTGACTC R:CCTCTCTCACCTGGAACTGC |
(TTC)4 | 60 °C | 3 | 157–163 | 0.62 | 0.30 | 0.50 |
| 17 | BrPe0037 | F:TGATAATGCAGCGAAAGAGC R:TCACACTCCATTTGCTCTGC |
(TG)6 | 60 °C | 3 | 227–231 | 0.61 | 0.20 | 0.49 |
| 18 | BrPe0010 | F:GAAGAAAAAAGGGCTTG R:GTTAGGGTTTGGAGGA |
(TC)9 | 55 °C | 3 | 200–204 | 0.60 | 0.40 | 0.48 |
| 19 | BrPe0001 | F:GTTGAGAGGATTGTGTTTG R:ATGGTAGAGGAGGAGAGA |
(CT)14 | 55 °C | 3 | 143–157 | 0.56 | 0.14 | 0.46 |
| 20 | BrPe0012 | F:AGAGAGAGAGAGAGAG R:ACATCATACTCCTCATCC |
(AG)8 | 55 °C | 3 | 214–218 | 0.58 | 0.13 | 0.45 |
| 21 | BrPe0008 | F:TTTTCAGCCTCCACTCTT R:TACACCACCAACACTCAC |
(AG)9 | 55 °C | 3 | 264–274 | 0.57 | 1.00 | 0.44 |
| 22 | BrPe0025 | F:CAAGGAACCAGAACAAGAAGAA R:GAAGAACAAGCCAGCCCA |
(GA)6 | 55 °C | 3 | 114–126 | 0.57 | 0.11 | 0.44 |
| 23 | BrPe0039 | F:GCTGCTCCACTGTGAATGTC R:AACCTAGCCCCGTCACAGTA |
(AT)6 | 60 | 3 | 193–203 | 0.57 | 0.10 | 0.44 |
| 24 | BrPe0050 | F:TCAAGGGTATCTTTGGTGCTG R:AGCTTCAGCGAGACAAAACC |
(TG)7 | 60 °C | 3 | 197–205 | 0.56 | 0.20 | 0.44 |
| 25 | BrPe0013 | F:GATCGAGGTGAGGTACTG R:GGTTTGGCTTTAATGGAGG |
(AG)8 | 55 °C | 2 | 169–171 | 0.53 | 0.00 | 0.38 |
| 26 | BrPe0020 | F:TAAAGCATCAGGTCAG R:TAGATAGATTTGACGGG |
(GT)7 | 55 °C | 2 | 295–297 | 0.53 | 0.00 | 0.38 |
| 27 | BrPe0034 | F:CCTGTGGTGAAAATGGAACC R:GAGCCCTGGACTGACACATT |
(CT)15 | 60 °C | 2 | 217–227 | 0.56 | 1.00 | 0.38 |
| 28 | BrPe0049 | F:GGGAATCAAAACCATGCAGT R:CTCCCAGCTTCCACTCACTC |
(TA)9 | 60 °C | 2 | 189–191 | 0.53 | 0.11 | 0.38 |
| 29 | BrPe3012 | F:CGCCCTTTCTGAAGATAATCC R:GCAATGCTAAGAAGGCCAAG |
(TCT)4 | 60 °C | 2 | 181–183 | 0.53 | 0.11 | 0.38 |
| 30 | BrPe0018 | F:TCCTTCCTTCTCCTCC R:ACACTTGTCTCTCATCT |
(CT)7 | 55 °C | 3 | 135–149 | 0.43 | 0.30 | 0.37 |
| 31 | BrPe0022 | F:GGCATAGAAGTGGAAGGG R:GGAAGGGAAGTGAAGGGA |
(AG)7 | 55 °C | 2 | 98–104 | 0.51 | 0.20 | 0.37 |
| 32 | BrPe0047 | F:TGGGCCATTTCTTTTCTCTC R:GAATCCTGCATGAGTTGAGGA |
(CT)9 | 60 °C | 2 | 186–192 | 0.48 | 0.30 | 0.35 |
| 33 | BrPe3014 | F:CGGAAGCGTGCTCATAAAGT R:AAGCCTGTGAGGTTGATTCG |
(AGA)5 | 60 °C | 2 | 218–220 | 0.48 | 0.30 | 0.35 |
| 34 | BrPe0007 | F:AAAGCCCAGATGAAGTGAA R:GGCTCCAATCAGAAGTGT |
(AG)9 | 55 °C | 2 | 177–179 | 0.40 | 0.50 | 0.31 |
| 35 | BrPe0027 | F:TCCAATCTTCTCAACC R:CAAACTAGTAAACCCC |
(TA)6 | 46 °C | 3 | 97–101 | 0.35 | 0.20 | 0.30 |
| 36 | BrPe3027 | F:CCAAAATGCCCAAAATGTCT R:GTCCGTGAGGAGATGTCGAT |
(GGT)4 | 60 °C | 3 | 178–202 | 0.35 | 0.40 | 0.30 |
| 37 | BrPe0019 | F:AAAGAGAAGGATGGATG R:AAAAAGGACGAGGAAGA |
(TC)7 | 55 °C | 2 | 210–214 | 0.36 | 0.14 | 0.28 |
| 38 | BrPe0044 | F:GGACGCTAAGAGACCCATTG R:TAAAAGCCCCACTTGCAATC |
(TA)6 | 60 °C | 2 | 217–219 | 0.33 | 0.38 | 0.26 |
| 39 | BrPe0016 | F:TGGTTGGTGGGTCTTGT R:CTCTTTCCTCTCTCTCTCTCT |
(AG)7 | 55 °C | 2 | 277–279 | 0.21 | 0.22 | 0.18 |
| 40 | BrPe0045 | F:CGCTTCCACTTTACCAGCTC R:GACCAACAACAGGCACAATG |
(GT)8 | 60 °C | 2 | 183–185 | 0.21 | 0.22 | 0.18 |
| 41 | BrPe0011 | F:GTTCTACTCCCTCATT R:CTTCTTAACATCCCCA |
(CT)8 | 53 °C | 2 | 74–80 | 0.19 | 0.20 | 0.16 |
| 42 | BrPe0017 | F:TTGTCTCTCGGTTCTCT R:CAAACACAAAACCCCC |
(AG)7 | 55 °C | 2 | 86–90 | 0.19 | 0.00 | 0.16 |
| AVERAGE | 3.26 | 0.55 | 0.35 | 0.45 |
F: forward primer; R: reverse primer; Ta: annealing temperature; Ho: observed heterozygosity; He: expected heterozygosity; PIC: polymorphic information content