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. 2017 Jul 20;18:26. doi: 10.1186/s12860-017-0141-0

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 10 — Effects of Cladosporol A and doxorubicin treatment on the expression of apoptosis related proteins. (a) Representative images of immunofluorescence analysis of effects of the expression of; antiapoptotic Bcl-2 and pro-apoptotic Bax by confocal microscopy (using 40× oil immersion lens) in human breast cancer (MCF-7) cells treated with Cladosporol A for 24 h (b). Statistical analysis of the expression of Bcl-2 and Bax. The relative fluorescence intensity was compared to the control group. Data are mean ± S.D. of three similar experiments; statistical analysis was done with **p < 0.01 and ***p < 0.001. c Western blot indicating the increase in bax and decrease bcl-2 protein expression after Cladosporol A treatment in concentration dependent manner