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. 2017 Jul 20;18:26. doi: 10.1186/s12860-017-0141-0

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — a Cell cycle distribution in human breast cancer (MCF-7) cells treated with 5, 10, 20 μM of Cladosporol A. Data are expressed as the mean ± SD of three similar experiments. b Cladosporol A disrupts the microtubules of human breast cancer (MCF-7) cells. Cells were treated with indicated concentrations of Cladosporol A for 24 h. Immunocytochemical staining was conducted using anti α-tubulin antibody and Alexa Flour-555-labelled secondary antibody and nuclei were stained with DAPI (c). Analysis of p21 protein expression of human breast cancer (MCF-7) cells after 24 h of Cladosporol A (5, 10, 20 μM) treatment by immunofluorescence microscopy (d). Western blot indicating the increase in p21 protein expression after Cladosporol A treatment in concentration dependent manner