Fig. 7.

a Intracellular ROS level was measured by flow cytometry analysis using DCFH- DA after 24 h. Human breast cancer (MCF-7) cells were treated with indicated concentrations of Cladosporol A and 0.05% H₂O₂ and incubated with 5 μM DCFH-DA. b Detection of ROS by fluorescence microscope. After incubation with DCFH-DA and 0.05% H₂O₂, MCF-7 cells (2 × 10⁵/ml/well) were washed and examined by fluorescence microscope (40X). c MCF-7 cell growth inhibition in the presence of ROS scavenger (NAC) was also determined. The cells were treated with indicated concentrations of Cladosporol A in the presence or absence of NAC (150 μM)